Load the same volumes of the dna sample and the dna ladder. Readytoload 1 kb dna ladder ranging from 500 to 10,000 bp. Please have a look into the related application note for more information about ladder migration in different gel concentrations. Thermo scientific generuler 1 kb plus dna ladder, ready. Sigmaaldrich offers a number of directload 1 kb dna ladder products. Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis.
A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose. July 17, 2018 related products materials from biotium are sold for research use only, and are not intended. The ladder is composed of fourteen chromatographypurified. Thermo scientific ogeneruler 1 kb dna ladder, readyto. This protocol is a combination of routinely employed methods. Generuler 1 kb plus dna ladder 75 to 20,000 bp is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. A dna ladder is a solution of dna molecules of different. U of rochester biological supply ctr g8633 stock manager. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Generuler 1 kb plus dna ladder can be labeled radioactively with t4 polynucleotide kinase. Shop online for a wide selection of thermo scientific generuler 1 kb dna ladder perform sizing and approximate quantification of a wide range of. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Hyperladder 1kb with 1 % wv agarose gel, hyperladder 50bp with 1.
Product description this molecular weight marker contains 16 double stranded dna fragments in wide range of sizes from. Thermo scientific generuler 1 kb dna ladder 2500bp. Thermo scientific ogeneruler 1 kb dna ladder, readytouse. The ladder is composed of fifteen chromatographypurified. Product information thermo scientific generuler 1 kb. Pdf easy method for production of a homemade dna ladder. How to dilute dna ladders andersen lab, 2016 we currently have two different kinds of ladder in the lab.
The blue dye migrates with 35 kb dna fragments in a 1% agarose gel and has an excitation peak of 424 nm. Generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Thermo scientific generuler readytouse 1kb dna ladder. The dna ladder consists of proprietary plasmids which are. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Thermo scientific generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Thermo scientific generuler 1 kb plus dna ladder fisher.
Thermo scientific fermentas fastdigest restriction enzymes. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. The absence of nucleases is confirmed by a direct nuclease activity assay. Home products products laboratory consumables molecular markers and ladders fermentas generuler 1 kb dna ladder readytouse, 5 x 50 ug fermentas generuler 1 kb dna ladder readytouse, 5 x 50 ug.
The 1 kb dna ladder is a unique combination of a number of. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. The 1 kb dna ladder was not designed for precise quantification of dna mass, but can be used for approximating the mass of dna in comparably intense samples of similar size. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. Contents amount storage sm03 generuler 1 kb dna ladder, readytouse 250 5 x 50 g for 500 applications, 0. Please have a look into the related application note for more information about ladder. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to.
Product information thermo scientific generuler 1 kb dna ladder. Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Contents amount storage sm31 generuler 1 kb plus dna ladder. Comparison between 1 kb plus dna ladder and 1 kb dna ladder. Investigating the effects of dmso on pcr fidelity using a. Migration patterns of pageruler prestained protein ladder in different electrophoresis conditions. Thermo scientific generuler dna ladder 1 kb perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this mixture of chromatographypurified.
Fermentas, and nuclease free water new england biolabs, ipswich, ma, u. Thermo scientific ogeneruler 1 kb dna ladder, readytouse 25010,000 bp perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this 1 kb dna ladder, premixed with blue and yellow tracking dyes. Supplementary data should be considered for more accurate adjustment. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. Effect of dmso in the pcr on the pattern of restriction fragments generated by digesting the pcr product of lacz with various restriction enzymes. Thermo scientific fermentas fastdigest enzymes are. How can i prepare working solution with 1 kb dna ladder. Use the loading dye solutions supplied with every fermentas dna ladder.
Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Do not exceed the amount of electrophoresis tracking dyes used for sampleladder preparation. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis condition. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer.
Generuler 1 kb dna ladder, readytouse catalog number sm03, sm0314 pub. Thermo scientific generuler 1 kb dna ladder 2500bp, 0. Description thermo scientific generuler 1 kb dna ladder is. A dna ladder is a solution of dna molecules of different lengths used. A was added to eppendorf tubes to a final volume of 20. How to dilute dna ladders andersen lab, 2016 invitrogen 1. Contents amount storage sm31 generuler 1 kb plus dna ladder 250 5 x 50 g for 500 applications, 0. Thermo scientific generuler dna ladder 1 kb 11833963 afdrukken thermo scientific generuler dna ladder 1 kb perform sizing and approximate quantification of a wide range of doublestranded. Page 14 material safety data sheet prepared in accordance with eu directive 91155eec printed. For quantification, adjust the concentration of the sample to equalize it approximately with the amount of dna in the nearest band of the ladder. Easy method for production of a homemade dna ladder in every laboratory. Thermo scientific generuler 1 kb dna ladder perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this mixture of chromatographypurified individual. Thermo scientific generuler 1 kb dna ladder thermo fisher.
Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. The 1 kb and 3 kb bands contain more dna to provide internal orientation. Fermentas generuler 1 kb dna ladder readytouse, 5 x 50. Unstained and fluorescent dna ladders jena bioscience. Dna ladder 100 bp 1kb marker for agarose gel electrophoresis. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide. Straightforward procedure for laboratory production of dna ladder. Shop online for a wide selection of thermo scientific generuler 1 kb plus dna ladder perform sizing and approximate quantification of a wide range of. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restrict. Generuler 1kb dna ladder application of the substance the preparation. William smith general phone 2755731 molecular biology sales specialist. Making 1kb ladder working solution university of san diego.
Home products products laboratory consumables molecular markers and ladders fermentas generuler 1 kb dna ladder readytouse, 5 x 50 ug fermentas generuler 1 kb dna ladder readyto. Thermo scientific generuler 1 kb plus dna ladder, readytouse perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions. View more information about our dna stains technology. The ladder is a mixture of chromatographypurified individual dna fragments. Do not exceed the amount of electrophoresis tracking dyes used for sample ladder preparation.
For optimal resolution, the recommended agarose gel concentration is 0. How can i prepare working solution with 1 kb dna ladder supplied by promega. The dna includes fragments ranging from 25010,000 base pairs. Generuler 1 kb plus dna ladder thermo fisher scientific. Thermo scientific generuler 1 kb dna ladder, readytouse. Product information thermo scientific generuler 1 kb dna. Migration patterns in different electrophoresis conditions using the prestained protein ladder v.
Generuler 1 kb dna ladder thermo fisher scientific. Page 1 4 material safety data sheet prepared in accordance with eu directive 91155eec printed. Thermo scientific ogeneruler 1 kb dna ladder, readytouse 25010,000 bp perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this 1 kb dna ladder. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. Based on our protocol, any laboratory can make its own 100 bp dna ladder. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. Use the loading dye solutions supplied with every fermentas dna laddermarker, as these solutions contain equilibrated amount of tracking dyes which will not mask dna under uv light. Generuler 1 kb plus dna ladder catalog number sm31, sm32 pub. Shop online for a wide selection of thermo scientific generuler 1 kb plus dna ladder, readytouse perform sizing and approximate quantification of a wide. Readytouse 1 kb dna ladder and readytouse 100 bp dna ladder page 1 of 1 psf006 revised.
Thermo scientific generuler 1 kb plus dna ladder, readyto. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Generuler 1 kb dna ladder, readytouse thermo fisher scientific. Thermo scientific generuler 1kb dna ladder, readytouse.
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